ABSTRACT: The purpose of this research is to analyze the changes in the family and gender relationships among the residents of Itiguçu due to the change of their lifestyle and their occupier condition since the creation of the Ecological Station Juréia-Itatins (EEJI), state of São Paulo during three decades (from 1970 to 2000). These changes were caused not only for the station’s creation impact but also for the intensification of the community relationship with the urban environment (Peruíbe), for its proximity and for the frequency that relationships are established with the modern world through the region tourism, due to the existence of the Paraíso Fall. The focus on family and gender relationships, tries to apprehend the objective form, the sexual division and the underlying gender relationships and their meanings. The research is based on information obtained in field survey with direct observation and with interviews and/or with free speech, applied in several periods between 1998 and 2000, to the residents of Itinguçu, mainly the women. The analysis concludes that the family continues playing a fundamental role in the Itinguçu residents’life. It presents a decomposition in its tradicional form in order to the abandonment of family agriculture, besides the impacts of Ecological Station creation, above all in that concerns the ownership rights. The no agricultural practices, integrated into the subsistence agriculture, that grows progressively, more the introduction of "modern values" in the way of life, promote also the variation in family form organization.